This concern arises because MSCs has an estimated diameter of 2030m15,16. The IP technique is an easy technique to master. All reactions were performed in triplicated. Blood 118, 330338, doi: 10.1182/blood-2010-12-327353 (2011). prepared Figure 5. Zhang, B. et al. https://doi.org/10.1038/srep30696. Caplan, A. I. PubMedGoogle Scholar. In the meantime, to ensure continued support, we are displaying the site without styles 5B). Experiments with microspheres has showed that most of particle of this size would be clear out by the lung17. Google Scholar. For example, discharged accompany with stool or cleared out by lung microphage. Integrative genetic analysis identifies FLVCR1 as a plasma-membrane Select search scope, currently: articles+ all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources . The most efficient method to inject small animals is a two-person method where one holds the rodent and the other person injects the rodent at about 10 to 20 degrees in mice and 20 to 45 degrees in rats. Injections, also known as shots, deliver liquid medications, fluids, or nutrients directly into a person's body. 2020 Apr;17(4):511-522. doi: 10.1080/17425247.2020.1736551. Intraperitoneal injection carries the risk of causing internal injury or of misplaced injection into a blood vessel or an organ, particularly the large thin-walled cecum or urinary bladder. From whole-body disease recovery, the amount of MSCs colons engraftment, tissue histological evaluation as well as levels of cytokines and TSG-6. To obtain Li, Y. P. et al. Centers for Disease Control and Prevention. and JavaScript. In IP group, the MSCs existence can be detected at day 1, but gradually decreased with the time, GFP+ cells can be hardly identified in inflamed colon after day 3 (data not shown). Direct injection of sodium pentobarbital (referred to as euthanasia by . MeSH When searching "intraperitoneal injections" a majority of the results relate to the use of IP injections in murine or rat models in order to study the effect of some drug or chemical. In patients with peritoneal surface malignancies, perioperative IP chemotherapy has been used with good results. Comparison of colonic proliferation 7 days after MSCs injection. Mice were made diabetic by intraperitoneal injection of streptozotocin, which led to a 2- to 2.5-fold increase in plasma glucose. One study utilized IP injections to study pain in the abdomen after a hysterectomy when administering anesthetic continuously vs patient-controlled. Roddy, G. W. et al. 2020 May 9;12(5):439. doi: 10.3390/pharmaceutics12050439. PubMed One article reviews the injection of sodium pentobarbital to euthanize rodents intraperitoneally. Bethesda, MD 20894, Web Policies For the other inflammatory cytokines, such as IL-6, IL-1 and IFN-, the expression levels varied among the groups and IP delivery did not show evident advantages over others (Supplementary Figure S7). iQ SYBR Green Supermix and CFX96 (Bio-Rad) were used for template amplification with a primer for each of the transcripts examined. Journal of immunology (Baltimore, Md. Google Scholar. We notice that the mean density of collagen was lowest in IP group, however, IP group did not exhibit less collagen deposition than the AI group, probably due to the scatter points (Fig. A simple method for evaluating dengue vaccine - ScienceDirect Briefly, colonic segments were homogenized at 50mg/mL in phosphate buffer (50mM, pH 6.0) with 0.5% hexadecyltrimethylammonium bromide. The incidence and impact of IBD is increasing worldwide. : 1950) 180, 15981608 (2008). Schrepfer, S. et al. Saroglitazar suppresses the hepatocellular carcinoma induced by However, they found the migrated PCs was very few and these may attribute to: 1) the recipient are heathy rat. Evid Based Complement Alternat Med. n=3 per group. FoxP3+ cells were greatly increased in MSCs therapy. Statistical significance was determined using one-way ANOVAs or Students t tests. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5(+) stem cell. 2018 Jul;6(3):207-216. doi: 10.29252/beat-060304. The physiologic characteristic of the peritoneal cavity not only helps remove toxic metabolites from the body, but also provides a useful portal of entry in the body for several pharmacological agents. Some of the examples depict situations where IP injections are not ideal, while others prove the advantageous uses if this delivery method. In IV group, most of MSCs were trapped in the lung, liver and spleen, while the cell that immigrated to the colon was not too much. Would you like email updates of new search results? Article And the loss of MSCs in the colon may attribute to many reasons, such as, wash out, cell death, or even rejection via the innate immune system. PDF Recommended Dose Volumes for Common Laboratory Animals - IQ Consortium Vishnubalaji, R., Al-Nbaheen, M., Kadalmani, B., Aldahmash, A. In the meanwhile, the colonic homogenates were collected and colonic IL-10, TNF- were measured by ELISA assays. Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. Buy cheap Viagra Plus online no RX - Effective online Viagra Plus ADS Lu, Z., Wang, J., Wientjes, M. G. & Au, J. L. Intraperitoneal therapy for peritoneal cancer. Google Scholar. The .gov means its official. A Simple Mouse Model for the Study of Human Immunodeficiency Virus Here, we also compare the expression of FoxP3, which is frequently used to quantitate Treg cells. The Journal of clinical investigation 117, 258269, doi: 10.1172/jci29159 (2007). It is more often applied to animals than to humans. Understanding when intraperitoneal injections can be utilized and in what applications is beneficial to advance current drug delivery methods and provide avenues for further research. Here, three different MSCs delivery routes: intraperitoneal (IP), intravenous (IV) and anal injection (AI) were compared on DSS-induced colitic mice model. X.W., Y.H., Y.N. The rate and amount of drug transfer in the peritoneum are dependent on several factors. Multiple Intravenous Transplantations of Mesenchymal Stem Cells To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Wang, M., Liang, C., Hu, H. et al. PubMed The optimum route of administration is an importance clinical issue not only for chemical drug, but also for stem cytotherapy. Solid lipid nanoparticle delivery of rhynchophylline enhanced the efficiency of allergic asthma treatment via the upregulation of suppressor of cytokine signaling 1 by repressing the p38 signaling pathway. Of the time points we tested (6, 16, 24 h) our results showed that a 16-h post-intraperitoneal injection image scan provided the best contrast in mice regardless of age or disease burden. This allows researchers to observe the effects of a drug in a shorter period of time, and allows them to study the effects of drugs on multiple organs that are in the peritoneal cavity at once. Federal law restricts this drug to use by or on the order of a licensed veterinarian. Cells, tissues, organs 169, 1220 (2001). Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone. Castelo-Branco, M. T. et al. Ulcerative colitis (UC) and Crohns disease (CD) are most common conditions in IBD. Most recently published study by Scaldaferri et al. Rodent (large / mouse) oral (intragastric), intravascular administration (tail vein injection, intraperitoneal injection, intramuscular injection and subcutaneous injection) Intragastric administration and oral taking of non-rodent (dog / monkey) solution and intravenous injection of extremities. Intraperitoneal injections may also allow too-rapid entry of bacteria or LPS into the blood. Intraperitoneal Injection of Oncolytic Viruses H101 for Patients With Lateralized activation of the insular cortex is also observed in adult . Disclaimer. . [19] It was concluded that IP administration should not be used over intravenous therapy due to high radiation absorption in the intestines. Currently, two types of stem are used for the treatment of IBD, hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs). Condition. Summary. Intraperitoneal injection of lithium chloride induces lateralized The AI and IV showed no significant intergroup difference, though AI had lower score (Fig. After 5 days of DSS contained water, all the subject mice showed strong positive test of fecal occult blood. seem to have found the key drug of MSCs in the treatment of colitis, TSG-647. Ng, S. C. et al. The peritoneal micromilieu commits B cells to home to body cavities and the small intestine. & Prockop, D. J. Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-kappaB signaling in resident macrophages. Opioids can produce peripheral analgesic effects by activation of opioid receptors on sensory nerves. (A) The colonic mRNA expression of TNF- and IL-10 was analyzed by RT-PCR; (B) cytokine contents in colonic protein extracts were determined by ELISA; n=6 mice/group; n=3 for the control group (C) Serum TSG-6 level in different groups measured by ELISA. Gastroenterology 149, 163176 e120, doi: 10.1053/j.gastro.2015.03.013 (2015). Samples were centrifuged at 30,000g for 20minutes and stored at 80C until cytokine determination. Free DiR and healthy mice (without colitis) were served as control. [6] In 1968, melatonin was delivered to rats intraperitoneally in order to study how brain serotonin would be affected in the midbrain. Mice are usu The Journal of infectious diseases 198, 456464, doi: 10.1086/590112 (2008). Intraperitoneal Drug Administration - ScienceDirect Kushnir, C. L., Fleury, A. C., Silver, D. F. & Spirtos, N. M. Intraperitoneal Catheter Placement: The Hammock Technique. PubMed The medium was refreshed every 23 days and third- and fourth-passage cells were used for all experiments. Injection of substances into peritoneum (body cavity), Current Drugs Delivered Intraperitoneally, "Why i.p. CAS But this study at least pointed out the possible migration route for cells by intraperitoneal injection. Muller-Ehmsen, J. The DiR dye alone was served as control. Euthanasia Process | American Association of Feline Practitioners Choi, H., Lee, R. H., Bazhanov, N., Oh, J. Y. Intraperitoneal injection of a substance in the mouse. Lee, R. H. et al. An example of how intraperitoneal injections can be optimized is depicted in a study where IP injections are used to deliver anesthesia to mice. In order to effectively administer drugs through IP injections, the stomach of the animal is exposed, and the injection is given in the lower abdomen. Colitis showed varying degrees of alleviation after three ways of MSCs transplantation and the IP injection showed the highest survival rate of 87.5% and displayed the less weight loss and quick weight gain. showed that incubation of human monoclonal IgG aggregates in plasma for 24 hrs resulted in alteration of the total number of aggregates, led to different aggregate size and changed their . Future Oncol 6, 16251641, doi: 10.2217/fon.10.100 (2010). Tyndall, A. High Pressure Nebulization (PIPAC) Versus Injection for the Intraperitoneal Administration of mRNA Complexes. Ceelen W, Braet H, van Ramshorst G, Willaert W, Remaut K. Expert Opin Drug Deliv. Study showed that genetically modified MSCs to increase CXCR-4 expression will lead to an increase of MSCs migration to intestinal with radiation enteritis and then resulted in disease improvement24. It has been reported that a large dose of exogenous IGF-I administered by injection has various side-effects, including alteration of circulating hormone profile and suppression of growth performance in growing pigs (Dunaiski et al., 1995). However, no significant TSG-6 difference was found between AI and IV groups. Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Kurtz, A. Mesenchymal stem cell delivery routes and fate. PDF Chris Quigley Essentials Full Spectrum Curriculum (2023) To compare the Ki-67 expression (one of the markers of cell proliferation) among the groups, the colon tissues were collected on day 7 (Fig. Treatment of dextran sodium sulfate-induced experimental colitis by adoptive transfer of peritoneal cells. Horizontal bars represent medians, boxes represent the 25th and 75th percentiles. The accessibility of the therapeutic cells to their expectable target site is strongly dependent on the route of administration used. A similar increase in mouse cytokines in the peritoneum was seen after IP injections of human fibroblasts. ISSN 2045-2322 (online). Intraperitoneally applied drug forms are supposed to be located in the peritoneal cavity. Method. It means IP injection is more feasible and reasonable for clinical settings. The number of therapeutic cells that can migrate and colonize at the injury site is a decisive prerequisite for the success of cytotherapy. Our work used marrow-derived MSCs but not adipose-derived ones. These early uses of Intraperitoneal injections provide good examples of how the delivery method can be used, and provides a base for future studies on how to properly inject mice for research. Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy. Blood 113, 4657, doi: 10.1182/blood-2008-04-154138 (2009). The fluorescence imaging disclosed higher intensity of engrafted cells in inflamed colon and the corresponding mesentery lymph nodes (MLNs) in IP and AI groups than the IV group. The expression level of TNF-, the most important pro-inflammatory cytokine, was markedly decreased in IP group, compared with AI (P=0.0174), IV (P=0.0019) and control group (P<0.001), respectively. The ELISA kit for TNFinduced protein 6 was used (CUSABIO Life science, China). PubMed Further control study comparing bowel signals on healthy mice and DSS mice after MSCs injection showed weaker NIR signal on healthy bowel in all three routes, which indicated fewer MSCs migration to the colon without colitis (Supplementary Figure S5). This study included unilateral SSNHL patients who were hospitalized in our department between . Abstract Intraperitoneal (IP) injection is frequently reported to be as effective as intravenous (IV) injection. Drug Deliv. In this study, three different MSCs delivery routes, intraperitoneal injection (IP), intravenous injection (IV) and anal injection (AI) were compared on DSS-induced colitis mouse model. PloS one 7, e33360, doi: 10.1371/journal.pone.0033360 (2012). . Influence of Systemic Administration of Coq10 Nanoparticles on Ischemia-Reperfusion Injury on Ovaries in Rat. Augello, A., Tasso, R., Negrini, S. M., Cancedda, R. & Pennesi, G. Cell therapy using allogeneic bone marrow mesenchymal stem cells prevents tissue damage in collagen-induced arthritis. If injecting a hazardous substance (biohazard, radiation hazard or chemical hazard), include precautions in the Animal Care Protocol for human safety (e.g. How to cite this article: Wang, M. et al. (A) Representative IHC images of each MSCs treatment group. The article overviews whether IP is the best option for euthanization based on evidence associated with welfare implications. C57BL/6 mice were brought from (68 weeks; SLACCAS, Shanghai, China), GFP transgenic mice ware brought from (The institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Peking Union Medical College, China). Gut 63, 588597, doi: 10.1136/gutjnl-2013-304636 (2014). Thank you for visiting nature.com. The need of intestinal resection in CD has remained stable8 and the colectomy rates in UC were still for 20% and 30% within 10 and 25 years of diseases duration9. We also evaluated serum TSG-6 in three MSCs administration route. The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. MSCs has been found to directly differentiate into end-stage cells to augment bone damages and repair cartilage defects38,39. In our study, we tracked the GFP+ MSCs colon colonization, but our results also showed the short stay of MSCs after injection. 2010 May;27(5):735-8. doi: 10.1007/s11095-009-0031-z. Interferon-gamma (IFN-gamma)- and tumour necrosis factor (TNF)-induced nitric oxide as toxic effector molecule in chronic dextran sulphate sodium (DSS)-induced colitis in mice. 2020 Jun 3;37(6):119. doi: 10.1007/s11095-020-02818-8. & Dittel, B. N. Isolation of mouse peritoneal cavity cells. Other than preformed at the site of injury, MSCs has showed ability to exert their therapeutic effects distally. Currently, therapy still largely relies on empirical and often implemented in a stepwise fashion: progressing through 5-aminosalicylate compounds, corticosteroids, immunomodulatory drugs and finally anti-TNF drugs7. of the needle exposed to assure 4-5mm injection depth. Google Scholar. Neutrophil infiltration was monitored by measuring MPO activity67. The levels of immunomodulatory cytokines were compared by real time-PCR and ELISA. Kalchenko, V. et al. Two early reports aroused the global interests of stem cell transplantation in IBD management. doi: 10.1002/14651858.CD005284.pub3. Kyriakou, C., Rabin, N., Pizzey, A., Nathwani, A. Tests were read by an independent observer (Cong Liang) and scored 05 as shown by the color indicators provided by the manufacturer. In this study, the intraperitoneal injection was demonstrated as the superior way for MSCs administration in the setting of colitis amelioration. Slider with three articles shown per slide. After centrifuge, the cells were certificated and transferred to MSC expansion culture medium consisting of a-minimum essential medium (a-MEM; Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 100g/mL penicillin/streptomycin. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. While this route is an easy to master, quick, suitable for chronic treatments and with low impact of stress on laboratory rodents, there is a common concern that it may not be an acceptable route for drug administration in experimental studies. But how these miracle cells reach to the lumen and by which possible migration route? Language links are at the top of the page across from the title. Ahmadian Kia, N. et al. Very few reports pay attention to factors applied in the clinic, such as injection or exposure routes, which has highly impact on stem cell therapeutic efficiency for IBD12. Cite this article. evaluated the therapeutic efficacy of different MSCs sources and delivery routes in experimental emphysema37. [citation needed], In humans, the method is widely used to administer chemotherapy drugs to treat some cancers, particularly ovarian cancer. Gut epithelium is intact and lacks cytokines or chemokines attraction; 2) most of PCs are immune cells, such as B cells (5060%), macrophages and mast cells (30%) and T cells (510%)28. In addition, the effects of different dosing modalities (e.g., oral administration and intraperitoneal injection) on the . 2014ZX09508002). Horwitz, E. M. et al. These mice were then randomized into 3 groups receiving different MSCs giving therapies. Then, the bone marrow was flushed out with 1mL syringe. PDF UBC ANIMAL CARE COMMITTEE TECH 10a Intraperitoneal Injection in the Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? From two streams of research, experimental and clinical, stem cell therapy showed promising results. There was a dose-dependent decrease in AGT activity in liver tissues of CD-1 mice to 24% of basal levels after four hourly intraperitoneal injections of O 6-methylguanine (110 mg/kg). Journal of molecular neuroscience: MN 44, 178185, doi: 10.1007/s12031-010-9446-6 (2011). Because labeled cells are founded in Peyers patch of the gut and peripheral blood, they proposed the most likely routes might be by both lymph- and blood circulation. Liu, T. et al. It causes irritation of the eyes, skin, mucous membranes, and upper respiratory tract. 2021. Intraperitoneal administration allows quick reabsorption of large volumes of substances and is the preferred injection route for nonirritant, isotonic solutions [7,11]. Several medications such as antibiotics are given via the intraperitoneal (IP) route in PD patients to treat episodes of peritonitis. ***P<0.001; **P<0.01. gynecological and gastrointestinal cancers and has shown very promising results. [8], A good example of how intraperitoneal injections work is depicted through "The distribution of salicylate in mouse tissues after intraperitoneal injection" because it includes information on how a drug can travel to the blood, liver, brain, kidney, heart, spleen, diaphragm, and skeletal muscle once it has been injected intraperitoneally.[9]. This work is licensed under a Creative Commons Attribution 4.0 International License. PubMed National Cancer Institute. Intraperitoneal Route of Drug Administration: Should it Be Used in 3; Supplementary Figure S6). These findings may benefit clinic practice. Available at: http://www.cdc.gov/ibd/ (Accessed: 7th April 2016) (2014). In various preclinical models, saroglitazar has been shown to prevent & reverse symptoms of NASH. Ray, A. Makhija, S. et al. (CS), Intraperitoneal, Polymicrobial, and Antibiotics available under aCC-BY 4.0 International license. Article 81421003 and No. Estes, J. et al. Intra-abdominal adhesions can be a considerable source of morbidity and mortality. Interestingly, in some IP injection mice, GFP+ cells were observed in the epithelium of the inflamed colon. Taede et al. 4F,G). PDF Proper Method for Intraperitoneal Injections - PerkinElmer In mouse, the intraperitoneal and subcutaneous LD 50 values are 138 and 650 mg kg 1, respectively. Yui, S. et al. Article Real time-PCR and ELISA also demonstrate lower TNF- and higher IL-10, TSG-6 levels in IP group.